Journal: Frontiers in Pharmacology

Article Title: Toxicity Assessment of Wild Mushrooms from the Western Ghats, India: An in Vitro and Sub-Acute in Vivo Study

doi: 10.3389/fphar.2018.00090

Figure Lengend Snippet: (A) The effect of the extracts on the morphology of NCM460 cells as visualized under a phase contrast microscope. (B) IC 50 values of the extracts. (C) Dose response curves w.r.t. exposure to different concentrations of the mushroom extracts on the intestinal cells. Data presented are the means ± SD of results from three independent experiments. “#” represents significant difference with respect to AB at p < 0.05.

Article Snippet: The NCM460 (colon epithelial cells) and Chang's liver cell lines were obtained from National Centre for Cell Sciences (NCCS), Pune, India, and grown at 37°C in a humidified atmosphere of 5% CO 2 using MEM, supplemented with 10% FBS and antibiotic solution.

Techniques: Microscopy

Journal: ACS applied materials & interfaces

Article Title: Design, Synthesis, and Nanostructure-Dependent Antibacterial Activity of Cationic Peptide Amphiphiles

doi: 10.1021/acsami.8b17808

Figure Lengend Snippet: Cytotoxicity of designed PA against HaCaT, HCEC and HEK-293 and hemolytic activity against Human red blood cells.

Article Snippet: Immortalized Human Keratinocyte (HaCaT) cell line was donated by Dr. David Oupicky Lab (Center for Drug Delivery and Nanomedicine – UNMC) and Immortalized Human Colon Epithelial Cell (HCEC) line was donated by Dr. Robert Lewis Lab (Eppley Cancer Institute – UNMC).

Techniques: Activity Assay

Journal: Genes & Genomics

Article Title: Tumor suppressive effect of scavenger receptor class A member 5 overexpression in colorectal cancer by regulating PI3K/AKT/mTOR pathway

doi: 10.1007/s13258-021-01139-3

Figure Lengend Snippet: Scavenger receptor class A member 5 (SCRAR5) expression in colorectal cancer (CRC) cells. The mRNA and protein levels of SCRAR5 in CRC cell lines (HT29, HCT116, SW480, and SW620) was significantly lower than that in normal HCoEpic cell line by qPCR ( A ) and western blotting ( B ), respectively

Article Snippet: Human normal colonic epithelial cell line HCoEpic and four CRC cell lines, including SW620, SW480, HT29, and HCT116, were purchased from Nanjing KeyGen Biotech.

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Identification of novel autophagy-related lncRNAs associated with a poor prognosis of colon adenocarcinoma through bioinformatics analysis

doi: 10.1038/s41598-021-87540-0

Figure Lengend Snippet: The qPCR result of seven lncRNAs in five COAD and normal colon mucosal epithelial cell lines. ( A ) qPCR result of AC027307.2; ( B ) qPCR result of AC073611.1; ( C ) qPCR result of MIR210HG; ( D ) qPCR result of AL161729.4; ( E ) qPCR result of LINC01063; ( F ) qPCR result of AC156455.1; ( G ) qPCR result of PCAT6.

Article Snippet: Normal human colon mucosal epithelial cell line (CCD-18Co) and the human COAD cell lines (SW480, LS174T, HCT116, DLD-1 and HT29) were purchased from iCell (Shanghai, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Fragmentation of Human Neutrophil α-Defensin 4 to Combat Multidrug Resistant Bacteria

doi: 10.3389/fmicb.2020.01147

Figure Lengend Snippet: HNP-4 1 – 11 and HNP-4 1 – 11 mod show only minor cytotoxic and hemolytic activity at high concentrations. We investigated the cytotoxic activity of HNP-4 1 – 11 and HNP-4 1 – 11 mod against (A) CaCo2/TC7 or (B) HT29 MTX E 29 cells. We seeded 1500 cells per well and treated them after 24 h with different peptide concentrations. Living cells were determined after 96 h treatment using a CellTiter Glo2.0 assay. (C) Hemolytic activity on human erythrocytes of the peptides compared to 0.1% Triton-X treatment. (A,B) Results from three independent experiments with ±SEM are shown, and (C) Results from two independent experiments with ±SEM are represented.

Article Snippet: Experiments were conducted with the human colonic epithelial adenocarcinoma cell line CaCo2 subclone TC7 which was obtained from the Robert-Bosch-Hospital Stuttgart, Germany.

Techniques: Activity Assay